Faculty Profile

Eleftherios T. Papoutsakis

Unidel Eugene du Pont Chair

DBI Faculty Fellow


  • Doctorate – 1980 Purdue University
  • Masters – 1977 Purdue University
  • Bachelors – 1974 National Tech. Univ. of Athens

About The Papoutsakis Group

The focus of the Papoutsakis Group is to study and modify the regulatory networks that control key cellular programs such as stem-cell differentiation and plasticity or prokaryotic sporulation and stationary-phase phenomena. Its research contributes to the understanding of cellular processes of both fundamental and industrial interests-such as stem-cell bioengineering or the link between sporulation, solvent production and stress response in solventogenic clostridia-and involves interdisciplinary work in the areas of systems biology, metabolic engineering, DNA microarrays, computational genomics, stem-cell biology, and immunology. By combining the use of state-of-the-art microarray platforms and data analysis techniques with classic molecular techniques and genomic tools, the Papoutsakis Group unravels the exquisitely crafted interplay of cellular processes that allow the exploration of cells for beneficial applications from Regenerative Medicine to Industrial Biotechnology.

Select Publications 

For a complete listing of publications, please view the Full CV.

  1. Al-Hinai MA, Jones, S and Papoutsakis ET, “The Clostridium Sporulation Programs: Diversity and Preservation of Endospore Differentiation”, Microbiol. Mol. Biol. 79, 19, (2015)
  2. Whitaker WB, Sandoval NR, Bennett RK, Fast AG, Papoutsakis ET, “Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization”, Current Opinion in Biotechnology 33(10), 1016, (2015)
  3. Jiang, J, Woulfe, DS and Papoutsakis ET, “Shear enhances thrombopoiesis and production of megakaryocytic (Mk) microparticles that induce Mk differentiation of stem cells”, Blood 124, 2094, (2014)
  4. Al-Hinai MA, Jones, S and Papoutsakis ET, “sK of Clostridium acetobutylicum is the first known sporulation-specific sigma factor with two developmentally separated roles, one early and one late in sporulation”, J. Bacteriology 196(2), 287, (2014)
  5. Zingaro, KA, Nicolaou, SA, Yuan, Y, and Papoutsakis, ET, “Exploring the Heterologous Genomic Space for Building, Stepwise, Complex, Multicomponent Tolerance to Toxic Chemicals”, ACS Synthetic Biology 3, 476, (2014)
  6. Panuganti S, Papoutsakis ET, Miller WM, “Three-stage ex vivo expansion of high-ploidy megakaryocytic cells: Towards large-scale platelet production”, Tissue Eng. A 19(7-8), 998, (2013)
  7. Gaida, SM, Al-Hinai, M, Indurthi, D, Nicolaou, SA & Papoutsakis, ET, “Synthetic tolerance: three non-coding small RNAs, DsrA, ArcZ and RprA, acting supra-additively against acid stress”, Nucleic Acids Research 41, 8726, (2013)
  8. Wang Q, Venkataramanan, K, Huang, H, Papoutsakis, ET & Wu, CH, “Transcription factors and genetic circuits orchestrating the complex, multilayered response of Clostridium acetobutylicum to butanol and butyrate stress”, BMC Systems Biology 7, 120, (2013)
  9. Venkataramanan, K, Jones, SW, McCormick, KP, Kunjeti, SG, Ralston, MT, Meyers, BC, & Papoutsakis, ET, “The Clostridium small RNome that responds to stress: the paradigm and importance of toxic metabolite stress in C. acetobutylicum”, BMC Genomics 14, 849, (2013)
  10. Al-Hinai MA, Fast, AG and Papoutsakis ET, “A novel system for efficient isolation of double- crossover recombination mutants in Clostridium enabling markerless chromosomal gene deletions and DNA integration”, Appl. Env. Microbiol 78, 8112, (2012)

Patents (h3)

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Awards (h3)

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Research Areas

Synthetic biology and metabolic engineering of microbial systems for biofuels and chemicals production; bioengineering of stem cells and hematopoiesis; experimental genomics and computational biology.

Office: 307 CLB
Phone: 302-831-0642

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